The Jacksonville Regional Chamber of Commerce compiled a list of the top area employers. The top 60 were published Friday, Monday and Tuesday and the next 20 are below. The Daily Record will continue to publish the top 100 in a series of reports this week. The chamber said the information is as of 2010 and will be updated next year. Employers might have added or cut jobs since the information was compiled.
| Rank | Company | City | Type of business | Employees |
| 61. | Stein Mart Inc. | Jacksonville | Retail, corporate headquarters | 1,000 |
| 62. | W.W. Gay Mechanical Contractor | Jacksonville | Mechanical contractors, corporate headquarters | 1,000 |
| 63. | Wolfson Children’s Hospital | Jacksonville | Hospital | 1,000 |
| 64. | Duval County Health Department | Jacksonville | Public health department | 900 |
| 65. | Florida Department of Transportation | Jacksonville | State transportation agency | 900 |
| 66. | GCA Services Group Inc. | Jacksonville | Building maintenance services | 900 |
| 67. | Landstar System Inc. | Jacksonville | Transportation logistics | 900 |
| 68. | Prudential Financial | Jacksonville | Insurance | 900 |
| 69. | Publix Super Markets warehouse | Jacksonville | Regional supermarket distribution center | 870 |
| 70. | Coggin Automotive Group | Jacksonville | Automobile dealers | 850 |
| 71. | Allstate Workplace Division | Jacksonville | Insurance headquarters | 850 |
| 72. | Baptist Medical Center Beaches | Jacksonville Beach | Hospital | 800 |
| 73. | Jacksonville District U.S. Army Corps of Engineers | Jacksonville | Federal government, engineers | 800 |
| 74. | NorthgateArinso (former Convergys) | Jacksonville | Human resources consulting services | 800 |
| 75. | Ring Power Corp. | St. Augustine | Construction equipment distribution, sales and service | 800 |
| 76. | Florida Hospital Flagler | Palm Coast | Hospital | 780 |
| 77. | ING | Jacksonville | Benefits and human resources outsourcing | 750 |
| 78. | Jacksonville Transportation Authority | Jacksonville | City transportation | 750 |
| 79. | Community Hospice of Northeast Florida | Jacksonville | Care for terminally ill children and adults | 700 |
| 80. | Fidelity Investments | Jacksonville | Investments | 700 |