The Jacksonville Regional Chamber of Commerce compiled a list of the top area employers. The top 60 were published Friday, Monday and Tuesday and the next 20 are below. The Daily Record will continue to publish the top 100 in a series of reports this week. The chamber said the information is as of 2010 and will be updated next year. Employers might have added or cut jobs since the information was compiled.
| Rank | Company | City | Type of business | Employees |
| 81. | Interline Brands | Jacksonville | Wholesale distributor | 700 |
| 82. | Securitas Security Services USA | Jacksonville | Security guards | 700 |
| 83. | St. Johns River Water Management District | Palatka | Water preservation and management | 700 |
| 84. | Duval County Jail | Jacksonville | City government, correctional institution | 690 |
| 85. | Florida School for the Deaf & the Blind | St. Augustine | School | 670 |
| 86. | Nassau County | Fernandina Beach | County government | 670 |
| 87. | Wal-Mart Food Distribution Center | Macclenny | Grocery warehouse | 660 |
| 88. | Medtronic | Jacksonville | Surgical products | 650 |
| 89. | Ponte Vedra Inn & Club | Ponte Vedra Beach | Hotel, resort | 650 |
| 90. | Florida Times-Union | Jacksonville | Newspaper publishing | 640 |
| 91. | SunTrust Mortgage | Jacksonville | Banking | 640 |
| 92. | Amelia Island Plantation | Amelia Island | Resort, real estate | 630 |
| 93. | Cypress Truck Lines | Jacksonville | Trucking, heavy hauling flatbed | 630 |
| 94. | The Haskell Co. | Jacksonville | Design construction firm, corporate headquarters | 630 |
| 95. | PGA Tour Inc. | Ponte Vedra Beach | Golf tournament planners | 620 |
| 96. | MSC Medical Services Co. | Jacksonville | Workers’ compensation services | 610 |
| 97. | HospitalityStaff | Jacksonville | Temporary services agency | 600 |
| 98. | Jacksonville Kennel Club | Orange Park | Race track, restaurant | 600 |
| 99. | PHH Mortgage | Jacksonville | Mortgage services | 600 |
| 100. | Prologistix | Jacksonville | Temporary services agencies | 600 |
| 101. | Putnam County Government | Palatka | County government | 600 |
| 102. | Unison Industries | Jacksonville | Aircraft parts, avionics manufacturer, corporate HQs | 600 |