The Jacksonville Regional Chamber of Commerce compiled a list of the top area employers. The top 40 were published Friday and Monday and the next 20 are below. The Daily Record will continue to publish the top 100 in a series of reports this week. The chamber said the information is as of 2010 and will be updated next year. Employers might have added or cut jobs since the information was compiled.
| Rank | Company | City | Type of business | Employees |
| 21. | AT&T | Jacksonville | Telecommunications, regional headquarters | 2,600 |
| 22. | Winn-Dixie Stores | Jacksonville | Grocery chain, corporate headquarters | 2,500 |
| 23. | Lender Processing Services | Jacksonville | Mortgage information technology processer, corporate headquarters | 2,400 |
| 24. | JEA | Jacksonville | Utility company | 2,300 |
| 25. | Catholic Diocese of St. Augustine | Jacksonville | Religious organization | 2,100 |
| 26. | Vistakon | Jacksonville | Optical products | 2,000 |
| 27. | Talagy | Jacksonville | Temporary services agency | 1,850 |
| 28. | Florida State College at Jacksonville | Jacksonville | State college | 1,800 |
| 29. | Gate Maritime Properties | Jacksonville | Port services | 1,800 |
| 30. | HCA-Memorial Hospital Jacksonville | Jacksonville | Hospital | 1,800 |
| 31. | Flagler County Public Schools | Bunnell | Public education | 1,750 |
| 32. | Aetna | Jacksonville | Health insurance | 1,700 |
| 33. | Putnam County School Board | Palatka | Public education | 1,680 |
| 34. | Flagler Hospital | St. Augustine | Hospital | 1,600 |
| 35. | University of North Florida | Jacksonville | State university | 1,540 |
| 36. | JM Family Enterprises/Southeast Toyota Distributors | Jacksonville | Regional automotive distributors | 1,500 |
| 37. | Marine Corps Support Facility – Blount Island | Jacksonville | Maritime prepositioning | 1,500 |
| 38. | State Farm | Jacksonville | Insurance and financial services | 1,500 |
| 39. | Swisher International | Jacksonville | Cigar and smokeless tobacco product manufacturing | 1,500 |
| 40. | Nassau County School Board | Fernandina Beach | Public education | 1,480 |