The Jacksonville Regional Chamber of Commerce compiled a list of the top area employers. The top 40 were published Friday and Monday and the next 20 are below. The Daily Record will continue to publish the top 100 in a series of reports this week. The chamber said the information is as of 2010 and will be updated next year. Employers might have added or cut jobs since the information was compiled.
Rank | Company | City | Type of business | Employees | | 41. | Gate Petroleum | Jacksonville | Petroleum products, corporate headquarters | 1,430 |
| 42. | Comcast Cable Corp. | Jacksonville | Cable television, broadband Internet services | 1,400 |
| 43. | EverBank | Jacksonville | Banking and mortgage services, corporate headquarters | 1,400 |
| 44. | Georgia-Pacific Corp. | Palatka | Pulp and paper mill | 1,400 |
| 45. | Internal Revenue Service | Jacksonville | Federal government | 1,330 |
| 46. | Crowley Maritime Corp. | Jacksonville | Marine transportation and logistics | 1,250 |
| 47. | Convergys | Jacksonville | Customer call center | 1,200 |
| 48. | Florida Department of Children and Families | Jacksonville | State government | 1,200 |
| 49. | Miller Electric Co. | Jacksonville | Electrical contractors | 1,200 |
| 50. | Orange Park Medical Center | Orange Park | Medical center | 1,200 |
| 51. | Northeast Florida State Hospital | Macclenny | Adult mental health hospital | 1,190 |
| 52. | YMCA of Florida's First Coast | Jacksonville | Health and fitness | 1,170 |
| 53. | Deutsche Bank | Jacksonville | Global technology and operations center | 1,100 |
| 54. | Palm Coast Data | Palm Coast | Magazine fulfillment, subscription processing | 1,100 |
| 55. | St. Johns County | St. Augustine | County government | 1,100 |
| 56. | VyStar Credit Union | Jacksonville | Credit union | 1,100 |
| 57. | BAE Systems/Atlantic Marine | Jacksonville | Ship builders, repair | 1,000 |
| 58. | Brooks | Jacksonville | Medical rehabilitation | 1,000 |
| 59. | Northrop Grumman Corp. | St. Augustine | Aircraft manufacturing | 1,000 |
| 60. | PSS World Medical Inc. | Jacksonville | Medical supplies provider, corporate headquarters | 1,000 |
